Objectives: This research was conducted as a cross-sectional descriptive study aimed at determining the existence of pain in the musculoskeletal system among office workers and the reasons for it. Material and Methods: The sample consisted of 528 office workers. Collection of data was achieved using a questionnaire prepared by the researchers in line with information from the literature. Results: The male and female office workers most frequently complained of pain in the lower back (55.1%), neck (52.5%) and back (53%). It was seen that out of the variables relating to the work environment, those which had the most significant effect on muscular-skeletal system pain were sitting at the desk for a long time without a break, working sitting on a chair that supported only the lumbar area and the arms, having the computer mouse at a distance from the keyboard, having the head inclined at 45° when working, working holding both forearms above the level of the desk, not taking exercise in daily life, and having a moderate or extremely stressful workplace (p < 0.05). Conclusions: The conclusion has been reached in this study that in order for office workers not to suffer musculoskeletal system pain, it is very important that the working environment should be ergonomically arranged and that various measures should be taken to ensure healthy life behavior. Int J Occup Med Environ Health 2018;31(1):91–111