1.191
IF5
0.947
IF
15
MNiSW
149.8
ICV
ORIGINAL PAPER
 
 

Assessment of DNA damage using chromosomal aberrations assay in lymphocytes of waterpipe smokers

Mohammad Azab 2, 3  ,  
 
1
Department of Applied Biological Sciences, Faculty of Science and Arts, Jordan University of Science and Technology, Irbid, Jordan
2
Department of Community Medicine and Pathology, Faculty of Medicine, The Hashemite University, Zarqa, Jordan
3
Department of Community Medicine and Pathology, Faculty of Medicine, The Hashemite University, P.O. Box 150459, Zarqa, 13115, Jordan
4
Department of Clinical Pharmacy, Faculty of Pharmacy, Jordan University of Science and Technology, Irbid, Jordan
5
Department of Biology, Faculty of Science, Yarmouk University, Irbid, Jordan
Int J Occup Med Environ Health 2012;25(3):218–224
KEYWORDS:
ABSTRACT:
Objectives: The aim of this study was to investigate the genotoxicity of waterpipe smoking in the lymphocytes of waterpipe smokers using chromosomal aberrations (CAs) assay. Materials and Methods: Fifty waterpipe smokers and 18 healthy nonsmokers volunteered to participate in the study. Additionally, 18 heavy cigarette smokers were recruited for comparison. Chromosomal aberrations (CAs) assay was used to evaluate DNA damage in the lymphocytes. Results: The results showed that similarly to cigarette smoking, waterpipe smoking significantly increased the frequencies of CAs (p < 0.01). In addition, the frequencies of CAs increased with more waterpipe use. Conclusions: Waterpipe smoking causes DNA damage to lymphocytes and the damage increases with more waterpipe use.
eISSN:1896-494X
ISSN:1232-1087