Objectives: The purpose of this study was to compare values of aerobic performance in the 1-mile run test (1-MRT) using different formulae. Material and Methods: Aerobic capacities of 351 male volunteers working for the Turkish National Police within the age range of 20-23 years were evaluated by the 1-MRT and the 20-metre shuttle run (20-MST). VO2max values were estimated by the prediction equations developed by George et al. (1993), Cureton et al. (1995) and Kline et al. (1987) for the 1-MRT and by Leger and Lambert (1982) for the 20-MST. Results: The difference between the results of the different formulae was significant (p = 0.000). The correlation coefficient between the estimated VO_2max using Cureton's equation, George's equation, Kline's equation and the 20-MST were 0.691 (p < 0.001), 0.486 (p < 0.001) and 0.608 (p < 0.001), respectively. The highest correlation coefficient was between the VO_2max estimated by the 20-MST and Cureton's equation. Similarly, the highest correlation coefficient (r = -0.779) was between the 1-mile run time and the VO_2max estimated by Cureton's equation. Conclusions: When analysing more vigorous exercise than sub-maximal exercise, we suggest that Cureton's equation be used to predict the VO_2max from 1-mile run/walk performance in large numbers of healthy individuals with high VO_2max. This research compares the use of 3 different formulae to estimate VO_2max from 1-mile run/walk performance in male law enforcement officers aged 20-23 years for the first time and reports the most accurate formula to use when evaluating aerobic capacities of large numbers of healthy individuals.