Objectives: The aim of this study was to estimate the level of exposure and average intensity of aggression towards particular professional groups of healthcare workers. Materials and Methods: Study participants (n = 1498) were employed at open and closed healthcare units within Podlaskie Voivodeship: 493 nurses, 504 midwives and 501 physicians. The MDM Questionnaire was applied. Results: Aggression originating from patients was experienced by 92% of nurses, 86% of doctors, and 74% of midwives examined. Aggressive behavior of co-working physicians concerned 55% of midwives, 54% of nurses and 40% of physicians. The highest average levels of patient aggression, ranging between 2.20 and 3.31, were reported by nurses. Conclusions: Nurses are the group most exposed to most aggression forms and sources. Physicians are least exposed to aggression, except for aggression manifested by patients.